FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n01_S2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n01_S2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26704979
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG370320.13867076997139746TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG60600.031.18810328
ACGTCTG62850.031.07222615
CGTCTGA64500.030.06806416
ACGTCCG65100.028.7545632
TCGTATG63800.028.4594345
CTCGTAT65050.028.29291744
GTCCGCA66700.028.0320134
GTCACGT69700.027.05163829
ATCGGAA76850.024.9485172
TCGGAAG79650.024.3255753
CCGCACA77700.024.29599236
TCACGTC79900.023.56989930
CGCACAT79600.023.34859337
TCCGCAC85000.022.36746835
CACGTCT88100.022.3199614
CACACGT93800.021.0116812
CAGTCAC93450.020.73063727
TCTCGTA90300.020.43138543
ACACGTC98500.019.98617713
GATCGGA103800.018.7771511
Produced by FastQC (version 0.11.5)