FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n01_S2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n01_S2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29053206
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTATG548810.18889825790654566TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG100200.033.54580715
GTCCGCA99200.032.571536
CGTCTGA104250.032.17784516
TCGTATG85150.031.31183445
CTCGTAT88650.030.53245444
ACCCGTC108750.029.99869332
ATCGGAA119550.027.9904692
TCGGAAG123400.027.2994543
CGCATCT113550.027.2862639
CACGTCT127550.026.63494914
TCCGCAT119400.026.60880337
CACACGT128400.026.24840412
CCGTCCG130000.025.6325934
ACACGTC133500.025.38043613
CCCGTCC131150.025.03057333
CAGTCAC133750.024.72938327
TCTCGTA111400.024.43852243
TCACCCG135450.024.1691330
AGTCACC138900.023.87679528
GTCACCC141250.023.16092929
Produced by FastQC (version 0.11.5)