FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n01_S2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n01_S2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28077251
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG2048710.7296690121123325TruSeq Adapter, Index 15 (97% over 40bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA320580.11417784454753066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG248150.040.4201345
CTCGTAT277300.038.41864844
GTCAGAA445350.036.64977336
AATCTCG361100.036.35196741
TGTCAGA459250.035.7742735
ACGTCTG466450.035.6399515
CGTCTGA466450.035.5773716
GAATCTC445950.035.11152340
TCTCGTA309200.035.00076743
GTCACAT473550.034.8035129
AGTCACA477550.034.54026428
CAGTCAC479950.034.4713527
AGAATCT460050.034.2609239
CACGTCT486400.034.2290414
ATGTCAG482750.034.158734
TCGGAAG489000.033.9672433
ACATGTC489950.033.69685732
ATCGGAA495600.033.5103532
CCAGTCA493650.033.4645526
ACACGTC501500.033.24775713
Produced by FastQC (version 0.11.5)