FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n01_S2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n01_S2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31596235
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG2885250.9131625967460996TruSeq Adapter, Index 14 (97% over 44bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA368990.11678290150709413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG331400.040.86431545
CTCGTAT358400.038.90952744
CGTATCT397900.038.5606739
CCGTATC403550.038.416338
TCCGTAT406300.038.28919637
AGTTCCG422850.037.4706334
TTCCGTA419400.037.3453836
GTTCCGT429500.036.5760935
TATCTCG404100.036.4646141
TCTCGTA390400.035.75480343
GTATCTC433500.035.29435340
ACGTCTG463400.034.58786815
CGTCTGA463650.034.54495616
GTCACAG466800.034.1018829
ACAGTTC468600.033.8444932
CACAGTT476150.033.42230231
CAGTCAC477250.033.38886327
TCACAGT476300.033.35052530
AGTCACA478550.033.2552128
CACGTCT487600.032.99582314
Produced by FastQC (version 0.11.5)