FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n01_S2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n01_S2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26203612
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG802570.3062822026215317TruSeq Adapter, Index 13 (97% over 40bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA349600.13341672132834206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG105700.036.46307445
CTCGTAT112450.034.1142544
ACGTCTG128300.034.08995415
CGTCTGA130600.033.52411316
TCGGAAG143700.030.395563
CACAGTC142250.030.38459631
CACGTCT145900.030.19355214
GTCACAG145650.029.8300729
ATCGGAA146250.029.7887062
AATCTCG135750.029.73503541
AGTCAAC146150.029.46630134
CAGTCAC149800.029.12433427
AGTCACA152650.028.53586628
CACACGT154650.028.52886612
ACAGTCA154800.027.97858232
TCTCGTA139250.027.67789543
ACACGTC161150.027.6015313
TCACAGT158050.027.44690530
CAGTCAA158550.027.11930833
CCAGTCA164750.026.49514426
Produced by FastQC (version 0.11.5)