FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17026371
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT7450.026.9111732
TCTCGGT5700.025.65527537
TAGATCT5650.025.4825533
GAGTGTA5950.024.95719528
AGTGTAG5800.024.82541829
GATCTCG6500.024.22594635
GTAGATC6000.023.99677332
GTGTAGA6350.022.6746530
AGATCTC7100.020.9096134
TGTAGAT7000.020.89041931
CTCGGTG8050.020.40350238
TTCCCTA3900.019.610968
GTGTAGG8900.016.93861417
TAGGGAA9350.016.84547820
GTAGGGA9150.016.47595419
TCGGTGG9350.016.36360239
TCGTGTA8950.016.34060315
TGTAGGG9400.016.03767218
TCCCTAC4700.014.8371239
ATCGGAA14300.014.1776852
Produced by FastQC (version 0.11.5)