FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n02_C2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n02_C2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28769521
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC29650.09.48355811
AATCGAA53650.09.4412335
GTACTCG55350.08.8700254
GGAAAAT113950.08.2448131
CACTCGT69900.08.23855535
GAATCGA40600.07.93224144
AGTACTC63400.07.9231443
GCGGAAA82300.07.8840931
ACTCGTG75450.07.871328436
CTCTATA74500.07.852118545
AAAAATC85550.07.8208362
TACTCGA59750.07.79917055
TCACTCG74900.07.718201634
GAAAAAT139750.07.6416231
CGTGACT26400.07.59456352
CGGGAAT22400.07.543491
AGAACGG179000.07.4750723
GAATTGA73650.07.3082114
AGCCTAG28050.07.29902225
AAAATCC84900.07.2963723
Produced by FastQC (version 0.11.5)