FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n02_C2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n02_C2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26884802
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC38600.012.52973411
CGTGACT26450.012.349742
TCAGGTA49600.011.111547
GCGTGAC35400.09.8647491
GTACTCG53650.09.6127194
CAGGTAG53200.09.55623158
AATCGAA48100.09.3137095
TAGAACA52300.09.24747912
GGTAGAA54500.09.16319910
GGAAAAT114900.09.0393771
CACTCGT51600.08.76277935
GTGACTC43000.08.7483373
GAATCGA42450.08.7005484
AGTACTC65650.08.5093133
AGATCAG94200.08.4631654
GCGGAAA64750.08.3160241
AAAATCC82100.08.3133673
TACTCGA61700.08.2823855
ACTCGTG56750.08.20572536
GAATTGA67050.08.1954254
Produced by FastQC (version 0.11.5)