FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n02_C2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n02_C2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31648473
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC45350.010.21829111
TCAGGTA57950.08.9281777
GTACTCG64350.08.7143894
GGAAAAT129300.08.27660751
GAATTGA80900.07.87814664
TACTCGA70200.07.8567845
CTCGAAT31250.07.71420241
GAAAAAT171000.07.61533451
CGTGACT29100.07.5866692
GGTAGAA62300.07.58262610
CAGGTAG63700.07.5572328
GAATCGA57500.07.520034
TAGAACA64100.07.510013612
AGTACTC78550.07.4560623
AATTGAC51850.07.42443135
AAAATCC95550.07.40254933
AGAACGG190850.07.34142883
GCGTGAC37400.07.16857151
AAAAATC96050.07.10662462
AACGGCC130550.07.0528115
Produced by FastQC (version 0.11.5)