FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n02_C2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n02_C2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23848606
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC32100.010.511641511
CGTGACT22250.09.0111912
TCAGGTA41900.08.9657527
GCGTGAC28550.08.4439641
CTCGAAT23250.08.4307261
GTACTCG43150.08.3509444
GCGGAAA55400.08.3370581
CACTCGT44650.08.31303335
AATCGAA44450.08.154145
AAAATCC71450.08.1340323
CAGGTAG41200.08.0806968
GAATCGA36800.07.89474774
GGAAAAT101550.07.85402061
GAAAAAT125550.07.78828241
CTCTATA47750.07.774740745
AAAAATC75150.07.76413352
TAGAACA42750.07.7350612
CGTTTAA130000.07.717646633
GGGAAAT54300.07.6346071
ACTCGTG49950.07.61151336
Produced by FastQC (version 0.11.5)