FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n02_C2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n02_C2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27014303
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAT108600.08.87953951
GGGAAAT59400.08.1930161
GTACTCG48300.07.92672734
CTCGAAT25600.07.65694951
GAATCGA43050.07.58554844
GGACTTA35700.07.5701624
TACTCGA48450.07.4808555
AGCCTAG27200.07.4442925
CGTGACT25100.07.359882
GTAGAAC32400.07.359505711
GAATTGA73550.07.2570124
AATCGAA52250.07.2383935
TCAGGTA41100.07.2246887
ATGTCTC134650.07.00142343
AAAATCC78850.06.9135453
TGTCTCC137900.06.8853544
GCCTAGA29150.06.791939726
AGTACTC58300.06.7617113
CGGAATC36650.06.76159762
CACTCGT56700.06.744710435
Produced by FastQC (version 0.11.5)