FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n02_C2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n02_C2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25799104
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGC328900.010.82929843
GGGGGCC117200.010.42448544
TCGGGGG81350.09.76393239
TTGGGGG105300.09.68001138
AGGGGCG83550.09.61187710
GTAGAAC39350.09.43249311
TCAGGTA49200.08.91570957
GGGGGCG311950.08.5470444
CCCGAAT18050.08.487641
GGGGCCG104900.08.45080645
GGAAAAT131900.08.3867181
TTCGGGG56400.08.37811238
TGGGGGG279300.08.1449439
GGGGAGA211150.07.863221622
AATCGAA53900.07.810365
CAGGTAG53200.07.61109738
AAAATCC87400.07.5257393
AAAGGGG111950.07.4748988
GGGGAAA125250.07.328543722
TAAATCG14200.07.2970493
Produced by FastQC (version 0.11.5)