FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n02_C2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n02_C2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23100454
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC33500.09.60231811
GGGGGGC147400.09.15861443
CCCGAAT17800.08.4804881
GGAAAAT109750.08.4167671
AATCGTA12200.07.93457275
TCAGGTA44800.07.9335017
AAAATCC73000.07.6835593
GAATCGA38750.07.49745044
GGTAGAA46200.07.44962210
GGAAGAT167650.07.44512841
TGGGGGG118800.07.424597739
GAATTGA64300.07.3903854
CAGGTAG45450.07.37459478
GGGGGCG147350.07.191936544
AATCGAA46200.07.11419535
AAAAATC76750.07.10322
CCGAATC28850.06.94962262
TAGAACA46200.06.914028612
AAATCCT80150.06.8280764
AAATCCA85850.06.79446174
Produced by FastQC (version 0.11.5)