FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n02_C2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n02_C2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28360681
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23750.09.9597032
AATCGAA51950.08.9703395
GTACTCG49900.08.8460734
GTAGAAC32100.08.82994311
GGGGGGC202550.08.6420443
GAATCGA42550.08.4157444
GGAAAAT115200.08.3900971
TACTCGA52050.08.0015675
CTCGAAT26000.07.88551661
GAATTGA73800.07.8122944
GCGTGAC34450.07.7825131
AGTACTC64100.07.62583073
AAATCGA56500.07.4141184
AATCGAC34350.07.3403265
AGAACGG167650.07.26907543
TCAGGTA42150.07.25827937
ATGTCTC101850.07.22363343
GAAAAAT130600.07.0557351
GGGAAAT63400.07.0361981
AATCGTA13150.07.0191125
Produced by FastQC (version 0.11.5)