FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n02_C2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n02_C2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24790833
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23950.011.1935512
GTAGAAC29450.09.31889611
GCGTGAC33200.09.16146851
ATGTCTC107950.08.7953943
TGTCTCC109400.08.5554244
GGAAAAT101350.08.5142121
GTACTCG49800.08.3210664
GATGTCT115100.08.17084942
AATCGAA45500.08.1143825
CTCGAAT21750.08.0798771
GATTCGA115950.08.070188534
GTGACTC38000.08.061993
TGATTCG116800.07.973664833
GAATCGA36800.07.8334424
AATTGAC41350.07.73100235
ATTCGAT123200.07.7236635
TACTCGA53750.07.53905735
GCATAGG34450.07.445894741
TCAGGTA38100.07.43932967
GAATTGA60750.07.41436864
Produced by FastQC (version 0.11.5)