FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17026371
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA12050.036.37854816
ACGTCTG12350.035.31075715
CACACGT13250.032.91238812
CACGTCT13400.032.87937514
ACACGTC13650.032.1124213
CCAGTCA10550.030.55390426
CAGTCAC9600.030.05588527
AGCACAC14700.029.66357810
GCACACG15200.028.54218911
CTCCAGT14550.025.40590124
ATCGGAA20250.024.5441232
TCGGAAG20500.024.1350983
CCGAGAC5900.023.68811429
GAGACCG6000.023.64832731
ACCGATC5550.023.50415434
AACTCCA17500.023.4339322
CGGAAGA21350.023.068884
GATCGGA21650.023.0653921
CTGAACT20150.021.97771819
GGAATGC7550.021.49388923