FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n01_C2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n01_C2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28769521
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA340790.11845522210814702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG41750.023.33401715
CGTCTGA43300.022.60265416
CGTAATC41550.022.36491838
CCGTACG43950.021.2468333
TCGTATG44750.019.30584145
CTCGTAT46100.018.7892944
CACCGTA50400.018.57180431
ACCGTAC53400.018.16025732
TCGGAAG60900.016.1107183
CACGTCT62350.016.02155114
ATCGGAA62600.015.4575462
CACACGT68000.014.75657512
GTAATCT69500.013.82382839
AATCTCG71200.013.30309441
CAGTCAC72850.013.2814927
TACGTAA73400.012.84417636
ACACGTC82000.012.62124113
TAATCTC75800.012.584825540
AGTCACC81600.012.51911428
ACGTAAT74500.012.44311837
Produced by FastQC (version 0.11.5)