FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n01_C2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n01_C2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26884802
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA330310.12286123587594211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG31650.027.65608833
GTTTCGG36900.023.96408534
ACGTCTG38250.023.88080215
AGTCACG38800.022.849328
CGTCTGA42400.021.80872516
ACGTTTC41100.021.514832
TTCGGAA41050.021.32211736
TCGTATG37550.019.77220345
TTTCGGA44200.019.75164835
CTCGTAT38350.019.41841144
TCGGAAG55250.017.2696673
ATCGGAA54450.017.192772
GTCACGT52800.016.96123729
TCGGAAT54150.016.37161837
CACACGT60500.015.95359812
CACGTTT56800.015.68697131
CACGTCT63200.014.9871614
CGGAATC60050.014.68814538
GGAATCT63500.014.31522839
CAGTCAC69400.013.35814527
Produced by FastQC (version 0.11.5)