FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n01_C2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n01_C2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31648473
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG373110.11789194379141137TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG68200.027.0179445
ACGTCTG77000.026.73551815
AGTCACG76350.026.52370828
CTCGTAT72300.025.8903444
CGTCTGA80550.025.52929716
GGCCTTA80950.024.9045636
CACGTGG83600.024.3037131
GCCTTAT87800.023.06405637
TATCTCG84650.022.77752741
GTCACGT89400.022.72746729
TCGGAAG99350.021.042733
ATCGGAA99650.020.9117012
ACGTGGC99500.020.71361732
CACGTCT100900.020.58110814
CACACGT102750.020.36385512
CCTTATC101700.019.71262438
TCACGTG105550.019.31368830
CAGTCAC108150.019.14088627
CTTATCT106000.019.0615539
ACACGTC117100.018.23339513
Produced by FastQC (version 0.11.5)