FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n01_C2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n01_C2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23848606
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA307720.1290306024595316No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG260380.10918038563763433TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG46300.027.45480545
AGTCACG48450.026.56459828
ACGTCTG50700.025.9600315
CTCGTAT48800.024.7574244
CGTCTGA53700.024.42595316
ACGTGAA54900.023.56568732
CACGTGA56200.023.22084831
CGTGAAA54950.023.13594233
AACGATC52100.023.06172438
TGAAACG57300.022.30391135
GTCACGT59650.021.8785529
GAAACGA60300.021.11963736
AAACGAT62650.020.32748237
TCGGAAG66500.019.9656413
CACGTCT68450.019.85275514
ATCGGAA67450.019.5843432
CACACGT71200.018.80169312
TCACGTG70300.018.59574530
CAGTCAC72300.018.39289927
ACACGTC82700.016.48634313
Produced by FastQC (version 0.11.5)