FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n01_C2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n01_C2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27014303
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG468820.17354510312555538TruSeq Adapter, Index 18 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT403650.1494208456905218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG79150.031.97881915
AGTCACG77800.031.87147728
CGTCTGA81750.030.96175616
GTCCGCA80400.030.70002634
TCGTATG79200.030.33945
ACGTCCG81050.030.31473432
CTCGTAT81050.029.59098444
TCGGAAG94700.026.8519173
GTCACGT93250.026.75980629
ATCGGAA95800.026.5436482
TCCGCAC93700.026.22232435
CACGTCT98700.025.82697914
CCGCACA95800.025.60054236
TCACGTC101750.024.32491130
CACACGT107350.023.74590312
CAGTCAC109450.023.0457127
ACACGTC113100.022.75748413
CGCACAT108850.022.57264137
TCTCGTA115100.021.1693943
CGGAAGA122350.021.1330874
Produced by FastQC (version 0.11.5)