FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n01_C2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n01_C2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25799104
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTATG4910711.9034420730270323TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG557200.042.3443545
CTCGTAT582650.041.4445944
GTCCGCA663600.040.32773236
TCTCGTA604150.039.76495443
CGCATCT656650.039.69569839
TCCGCAT673550.039.2609837
ACCCGTC691500.039.0846532
CCGTCCG694700.038.91101534
ACGTCTG709600.038.73539415
CGTCTGA710250.038.6809416
CCGCATC690700.038.26984838
CCCGTCC709200.038.12025533
CGTCCGC710400.038.07958235
TCACCCG711550.038.03502730
ATCGGAA730000.037.6982352
GTCACCC719150.037.66195729
CACGTCT733150.037.54331214
ATCTCGT653700.037.52518542
CACACGT733600.037.4682212
TCGGAAG734600.037.4529843