FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n01_C2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n01_C2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23100454
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG1118810.48432381458823276TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG143750.038.14142645
CTCGTAT155150.037.23852544
CGTCTGA233400.034.12423716
ACGTCTG236800.033.71978815
GTCAGAA237000.033.1237536
AATCTCG202350.032.9663441
TGTCAGA240400.032.73951335
TCTCGTA182100.032.24629243
GAATCTC240600.031.42845740
ATCGGAA254700.031.3745962
GTCACAT254000.031.12061729
CACGTCT257200.031.08026914
CACACGT257500.031.02658712
TCGGAAG258650.030.965063
CAGTCAC258750.030.7319427
AGTCACA260550.030.4505428
ATGTCAG260700.030.3973134
ACACGTC265650.030.16786813
AGAATCT254250.029.9559239
ACATGTC267300.029.68846332
Produced by FastQC (version 0.11.5)