FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n01_C2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n01_C2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28360681
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG2027500.7148982071340247TruSeq Adapter, Index 14 (97% over 44bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA324720.11449654541088065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG239800.040.31530845
CTCGTAT258450.038.0851244
CGTATCT276600.037.74393539
CCGTATC278400.037.70201538
TCCGTAT280900.037.47860737
AGTTCCG292650.036.45825634
TTCCGTA292800.036.08603336
TATCTCG285500.035.65088341
GTTCCGT302800.035.05776235
GTATCTC308300.033.75871740
TCTCGTA291250.033.75742343
CGTCTGA327100.033.09830516
ACGTCTG328250.033.0234715
GTCACAG332700.032.31411729
ACAGTTC334100.032.04973632
CAGTCAC339100.031.76395827
TCACAGT338000.031.66057230
AGTCACA340750.031.51109928
CACAGTT342900.031.2996831
CACGTCT349700.031.08794214
Produced by FastQC (version 0.11.5)