FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l07_n01_C2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l07_n01_C2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24790833
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA344090.13879727236273182No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG254680.10273152176855051TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG49450.026.75273515
CGTCTGA50750.026.37777116
TCGTATG48300.025.9920145
CTCGTAT48550.025.44110144
TCGGAAG65550.020.770033
ATCGGAA66050.020.4424462
AGTCAAC66350.020.21116834
CACAGTC66150.020.13650331
CACGTCT70600.019.43937714
GTCACAG69400.018.99971229
CACACGT72250.018.7151712
CAGTCAC73250.018.30827727
AATCTCG70950.018.17000441
TCACAGT76400.017.52357930
AGTCACA76950.017.42795828
TCTCGTA75400.016.88876543
ACACGTC82500.016.71720713
CAGTCAA85700.015.70078333
CCAGTCA86600.015.5116326
ACAGTCA90400.015.40652832
Produced by FastQC (version 0.11.5)