FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17677710
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT7300.030.2361032
TCTCGGT5700.026.83971437
AGTGTAG6000.025.50047329
GAGTGTA6200.024.67787628
TAGATCT6350.024.44649933
GTGTAGA6500.023.88505730
GTAGATC6600.023.52216332
CTCGGTG7650.020.88350938
TCGGTGG7450.020.53845639
AGCGGTT4900.020.19816210
GATCTCG7850.020.05815735
TGTAGAT7800.019.90348231
TAGGGAA8700.018.36417820
GTGTAGG8700.018.10552817
TTCCCTA4250.017.9947768
AGATCTC8500.017.99462534
GTAGGGA8900.017.95149819
TGTAGGG8950.017.59978718
TCGTGTA8800.017.38717815
CGGTTCG3000.016.49432612