FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n02_B2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n02_B2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25703187
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28100.013.0643712
GTAGAAC32500.010.45094311
GCGTGAC38150.010.3323271
GTACTCG58000.010.2104214
TACTCGA62900.09.376025
GAATCGA40050.09.2205594
TCAGGTA42100.09.13655957
AGTACTC66400.09.0894323
GTGACTC42450.08.9655933
CTCGAAT22400.08.6477881
TATCCAG64050.08.6453765
GAATTGA64150.08.5646394
GGAAAAT115900.08.2790561
ATTGACC42350.08.2327896
CTCTATA56050.08.06731145
AAAATCC80850.07.9941433
TCACACG26250.07.970285415
AATCGAA47800.07.95841745
AATTGAC44150.07.95356375
CACTCGT55700.07.75396835
Produced by FastQC (version 0.11.5)