FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n02_B2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n02_B2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27185770
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC49100.012.048408511
CAGGTAG60450.010.1212578
TCAGGTA63350.010.0130027
CTAATCG27000.010.0093553
TAGAACA60900.09.52896812
AATCGAG52250.09.3487635
TAATCGA36550.09.3030344
GAATCGA42250.09.2737964
GGTAGAA64950.09.07367410
AGGTAGA68550.08.8924969
GGAAAAT146800.08.7002721
GAATTGA71600.08.5229774
AATCGAA51600.08.2014175
CTCGAAT28450.08.0759521
AAAATCC95200.07.97226243
ATCTAAC19750.07.8585896
AATTGAC46400.07.76216175
AGATCAG114200.07.5718174
ATCAGGT115050.07.3708466
AAAAATC83350.07.18790342
Produced by FastQC (version 0.11.5)