FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n02_B2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n02_B2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28862940
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26050.011.326282
GTAGAAC40500.010.83021511
TCAGGTA50100.09.4734197
GTACTCG53750.09.2586384
GCGTGAC37500.08.8299291
TACTCGA57100.08.67266755
GGAAAAT117250.08.6451171
CTCGAAT24950.08.4864971
AATCGAA49150.08.4725545
GGTAGAA55100.08.28713110
GAATCGA45400.08.283124
AGAACGG178200.08.0504333
CAGGTAG57900.07.8087148
GAATTGA77050.07.65705874
TAGAACA57850.07.620771412
AGTACTC67100.07.5517493
AGGTAGA60550.07.4669629
ATGTCTC119000.07.430748543
AACGGCC121700.07.3798655
GGGAAAT66650.07.36762481
Produced by FastQC (version 0.11.5)