FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n02_B2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n02_B2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24566111
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC33950.09.60705711
GTACTCG42800.09.3649484
AATCGAA41950.08.8001975
TCAGGTA42850.08.4516527
TACTCGA45400.08.2802055
TGTCTCC102050.08.11297344
ATGTCTC101100.08.10054143
CGTGACT20700.08.0517042
GGAAAAT95100.07.982221
AGTACTC53200.07.91624643
GGTAGAA43600.07.738726610
AAATTGA85150.07.72195634
GAATTGA61450.07.69531774
AATCGGC17100.07.6350595
TAGAACA46050.07.42437912
GAATCGA39300.07.39139274
GATGTCT113250.07.3904142
ATTCGAT114150.07.38982735
GATTCGA112750.07.381605634
AAATCCA92350.07.36375244
Produced by FastQC (version 0.11.5)