FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n02_B2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n02_B2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27527704
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT29700.012.664372
GCGTGAC37200.011.3232121
GTAGAAC43650.010.66716911
TCAGGTA52150.09.48932657
GTACTCG59450.09.1283684
TACTCGA63600.08.6005985
GGAAAAT129000.08.573591
GGGAAAT57350.08.5230931
CCCGAAT17100.08.4305291
GTGACTC47300.08.3799443
CAGGTAG58900.08.2872698
AATCGAA54250.08.2572015
AGTACTC71300.08.1808763
AGCCTAG25900.07.99237425
TAGAACA58600.07.98394812
ATCTAAC14750.07.93009426
GGTAGAA58450.07.92768810
AAAATCC86150.07.81637673
AGATCAG98950.07.6463024
GAATTGA66300.07.6418234
Produced by FastQC (version 0.11.5)