FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n02_B2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n02_B2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26011748
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG70600.011.18676539
GGGGGGC274850.010.60118543
GTAGAAC38000.010.0630511
CGTGACT25050.09.3504592
GGGGGCC103250.09.26108244
TTCGGGG57750.09.19495438
TCAGGTA46300.09.1822727
AGGGGCG70650.08.97853410
CTCGAAT22800.08.8912121
AATCGAA50450.08.8787855
GGGGGCG257750.08.702805544
GTACTCG58700.08.63128954
GAATCGA37000.08.4594944
AGTACTC67150.08.2837253
TTGGGGG100300.08.05347138
GAATTGA64300.08.0196574
AAAGGGG94650.07.985248
GGGGGAA182600.07.91002521
TACTCGA63200.07.9067415
GGTAGAA51500.07.90569110
Produced by FastQC (version 0.11.5)