FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n02_B2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n02_B2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24969890
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG63900.010.5367364
GTAGAAC37650.010.51512311
AGTACTC69550.010.3943363
TACTCGA66950.010.1538375
AATCGAA42750.010.1097155
CGTGACT25250.09.9903022
TCAGGTA44000.09.611157
AATTGAC41500.09.1124475
GGAAAAT103450.08.9490481
CTCGAAT21800.08.6793711
GAATCGA39000.08.6608884
GAATTGA62450.08.5097214
ATGTCTC110250.08.48957243
GCGTGAC34800.08.2203441
ATTGACC43800.08.217076
TGTCTCC112800.08.17764944
GATTCGA117950.08.00979734
AGAACGG147050.07.9792413
GATGTCT119700.07.96973142
TCACACG22600.07.76434915
Produced by FastQC (version 0.11.5)