FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n02_B2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n02_B2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25748683
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG68150.011.81961339
GGGGGGC258350.011.295135543
CGTGACT22200.011.2611192
GTAGAAC31600.010.53515511
GGGGGCC100850.010.30643144
TAGGGGG19400.09.85832939
TTCGGGG58050.09.68981438
AGGGGCG72400.09.16543710
GGGGGCG245900.08.93878144
GAATCGA37200.08.8374824
TTGGGGG97300.08.579041538
ATTCGGG30250.08.47804237
TCAGGTA42000.08.4085957
AATCGAA46400.08.3926655
GGGGCCG87350.08.36992145
GGAAAAT114000.08.3576831
GCGTGAC34350.08.3277441
TGGGGGG207700.08.28726239
GAATTGA65250.08.2132514
AATTGAC43900.08.0502035
Produced by FastQC (version 0.11.5)