FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n02_B2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n02_B2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23455690
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC29150.010.5715411
CGTGACT22600.010.16509152
AATCGAA42600.09.7226985
GTACTCG50200.09.509594
CTCGAAT20350.09.0764551
TACTCGA53250.08.8349735
AGTACTC58100.08.7215093
GGAAAAT97200.08.7134151
GAATTGA60250.08.596084
GCGTGAC32700.08.4727461
TCAGGTA41200.08.0803647
GAATCGA34350.08.06321054
ATGTCTC92850.08.045073543
AAAATCC70500.07.9861263
GGTAGAA41300.07.84287310
GATTCGA96700.07.676434
GATGTCT101650.07.481420542
TGTCTCC99100.07.42379144
TAGAACA42800.07.410007512
TGATTCG100600.07.40249233
Produced by FastQC (version 0.11.5)