FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17677710
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA9950.034.56947716
ACGTCTG10150.034.32789615
CACACGT10900.032.1736612
CACGTCT11050.031.32976514
ACACGTC11300.031.03450613
AGCACAC12350.029.11605610
GCACACG12300.028.69438611
CAGTCAC9000.028.54768227
CCAGTCA9800.027.13708326
GAGACCG5700.026.08489631
CCGAGAC5750.025.47610729
CTCCAGT12200.024.75333624
ACCGATC5850.023.46358334
AACTCCA14350.023.38705822
TCGGAAG19600.022.4814573
ATCGGAA20200.021.8136942
CGAGACC7000.021.24622230
AGAGCAC17200.021.1668518
CTGAACT17100.020.766719
CGGAAGA21400.020.484634