FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n01_B2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n01_B2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25703187
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA303590.11811375764413962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG34450.021.94333515
CGTAATC34950.020.47264338
CGTCTGA37100.020.1942616
TCGTATG34650.019.0255545
CCGTACG37050.018.70465533
CTCGTAT37000.017.99973944
ACCGTAC40850.017.0735232
CACCGTA42300.016.43506831
TCGGAAG50050.015.108383
ATCGGAA49800.015.00348952
CACGTCT54850.014.31552514
CACACGT56650.013.74154312
GTAATCT57300.013.03701839
TACGTAA54000.012.91690736
ACGTAAT55400.012.7935637
CAGTCAC59600.012.75990827
AATCTCG61500.012.07299641
ACACGTC71000.011.53457313
AGTCACC71100.011.20239228
TAATCTC65900.011.09621940
Produced by FastQC (version 0.11.5)