FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n01_B2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n01_B2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27185770
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG29000.026.37968333
GTTTCGG34000.022.69905934
ACGTCTG37100.021.64945415
AGTCACG37750.020.6820328
CGTCTGA40700.019.5688616
TTTCGGA41150.018.59088735
TTCGGAA42150.018.3633536
ACGTTTC47550.016.3238632
CTCGTAT40200.016.28688444
TCGTATG38650.016.24136545
GTCACGT48650.016.04814529
ATCGGAA51400.015.7626012
TATGGGT43200.015.6766743
TCGGAAG53200.015.5675373
TCGGAAT53300.014.47964637
CGGAATC58100.013.51585438
ATATGGG53400.013.0193242
CACACGT63800.012.94206812
CAGTCAC68600.012.56189927
ACACGTC68800.012.29582513
Produced by FastQC (version 0.11.5)