FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n01_B2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n01_B2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28862940
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA315980.10947602704367608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG50300.024.19827315
AGTCACG52650.022.73470728
CGTCTGA54050.022.64446316
TCGTATG49300.021.81464445
GGCCTTA56100.021.45772236
CACGTGG56800.021.38960531
CTCGTAT53600.020.3584244
GTCACGT60250.019.90427429
GCCTTAT64700.018.5707637
TATCTCG64500.017.72030341
TCGGAAG71900.017.5282143
CACGTCT72550.017.27335414
ATCGGAA73300.017.2242222
ACGTGGC73850.016.72547532
CCTTATC76700.015.69463238
CACACGT82800.015.08072712
CTTATCT79750.015.00986139
ACACGTC87250.014.87884513
CAGTCAC83550.014.70355527
TCACGTG82650.014.700130
Produced by FastQC (version 0.11.5)