FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n01_B2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n01_B2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24566111
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA317020.12904769501367147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG38600.024.42327928
ACGTCTG39900.024.13357415
TCGTATG42650.022.57881545
CGTCTGA43250.022.05646116
CGTGAAA43750.021.60044333
CTCGTAT44450.020.44969644
AACGATC44700.020.43699338
CACGTGA46950.020.31804531
ACGTGAA48300.019.8433132
GTCACGT49550.019.34378229
TGAAACG50450.018.68746835
GAAACGA52500.018.08634236
TCGGAAG55800.017.4637343
CACGTCT57550.017.31866614
ATCGGAA56900.017.1658122
AAACGAT56650.016.80111337
CACACGT66500.015.19080312
CAGTCAC64250.014.98811427
TCACGTG68100.014.04116530
ACACGTC73700.013.92045513
Produced by FastQC (version 0.11.5)