FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n01_B2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n01_B2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27527704
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG44350.024.14851828
ACGTCTG45650.023.80467215
TCGTATG45000.022.8992645
CTCGTAT46500.022.20944
CGTCTGA49400.022.1345116
GTCCGCA48400.022.1285134
ACGTCCG52250.020.49638632
GTCACGT57950.018.79179629
TCGGAAG62250.017.8232353
ATCGGAA62000.017.7497792
CCGCACA61400.017.47996536
TCCGCAC64200.016.99797835
TCACGTC68100.015.99073130
CACGTCT71300.015.58824514
CGCACAT70950.015.127130537
CACACGT75900.014.91031412
CAGTCAC76950.014.32730827
TCTCGTA75900.013.93238143
ACACGTC86650.013.55382113
CCAGTCA88750.012.19421626
Produced by FastQC (version 0.11.5)