FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n01_B2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n01_B2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26011748
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTATG3462201.3310139710718403TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG392050.041.44082645
CTCGTAT411200.040.6874544
GTCCGCA476800.040.2016736
CGCATCT475800.039.14678239
ACGTCTG503650.039.0244815
CGTCTGA503950.039.0062416
TCCGCAT488750.038.66629437
ACCCGTC504750.038.32446732
TCTCGTA438550.038.17051743
TCACCCG513750.037.71570630
CACGTCT524700.037.5450814
ATCGGAA526200.037.5044482
TCGGAAG526250.037.487773
CCCGTCC516000.037.48735433
CAGTCAC519600.037.3262927
CCGTCCG520400.037.31797834
CGTCCGC520000.037.31617735
AGTCACC523250.037.06168728
CCGCATC510200.037.02317438
CACACGT533500.036.9089112