FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n01_B2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n01_B2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24969890
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG561100.2247106414966185TruSeq Adapter, Index 15 (97% over 40bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA365820.14650444995953127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG80850.033.78350445
CTCGTAT88300.033.07362444
GTCAGAA145050.028.63518736
ACGTCTG149250.028.32476615
AATCTCG126850.027.82917241
CGTCTGA152300.027.75774816
TGTCAGA151700.027.57273935
GTCACAT158700.026.39876629
GAATCTC156150.026.07996640
TCTCGTA115050.025.73576543
CAGTCAC164150.025.59082627
AGTCACA165000.025.4044528
AGAATCT162550.025.23413339
CACGTCT170200.024.97064614
ACATGTC172100.024.45979932
ATGTCAG172550.024.33242234
TCACATG175050.024.1641930
TCGGAAG175500.024.1468813
CACACGT176800.024.01303312
ATCGGAA177100.023.9160652
Produced by FastQC (version 0.11.5)