FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n01_B2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n01_B2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25748683
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG3253031.2633772375853165TruSeq Adapter, Index 14 (97% over 44bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA264310.10264991028861555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG367450.041.802745
CTCGTAT398050.039.6462144
CGTATCT439950.039.12432539
CCGTATC445750.038.81707838
TCCGTAT449300.038.6154737
AGTTCCG465000.037.8730534
TTCCGTA461500.037.72628836
TCTCGTA422100.037.51521743
TATCTCG442250.037.47463641
GTTCCGT471400.037.10104435
GTATCTC468850.036.64916640
ACAGTTC499400.035.32895332
GTCACAG503050.035.07025529
ACGTCTG510250.035.0539215
CGTCTGA510800.035.01685316
CACAGTT506500.034.842631
TCACAGT508250.034.65751330
CAGTCAC515050.034.4410727
ATCTCGT469500.034.40344242
AGTCACA517150.034.20121828
Produced by FastQC (version 0.11.5)