FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l06_n01_B2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l06_n01_B2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23455690
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG284960.12148864518588026TruSeq Adapter, Index 13 (97% over 40bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA268120.11430915057284607No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250950.10698896515088663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG52550.030.2265315
CGTCTGA54350.029.22559216
CTCGTAT48900.028.4809744
TCGTATG50300.028.4486845
TCGGAAG67600.023.6699733
ATCGGAA68550.023.1777942
CACAGTC69200.022.59632531
CACGTCT72900.022.15944114
GTCACAG72800.021.72727629
CACACGT75250.021.31796812
CAGTCAC75950.020.94468727
AGTCACA77850.020.34685128
TCACAGT79600.019.78595730
AGTCAAC80450.019.63355334
AATCTCG74850.019.32823241
ACACGTC85350.019.16429113
TCTCGTA73550.019.05804643
ACAGTCA88100.018.15732632
CCAGTCA90250.017.70081926
AGCACAC94700.017.46224810
Produced by FastQC (version 0.11.5)