FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18444811
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT4700.031.59219437
CACTCTT7850.028.6840422
AGTGTAG5700.025.66323329
TAGATCT5900.025.16987633
GTAGATC6000.024.75353832
GAGTGTA6200.023.59361828
GATCTCG6700.023.1671535
CTCGGTG6750.022.9998538
GTGTAGA6400.022.8561330
AGCGGTT4850.022.72601110
TCGGTGG7200.021.24957539
AGATCTC7350.020.81155834
TGTAGAT7350.019.9011831
TTCCCTA5250.019.7098758
TGTAGGG8450.019.17277718
GTGTAGG8400.019.01856217
TAGGGAA8650.018.72947320
TCGTGTA8650.018.4687915
AAGAGTG9300.017.66419826
GTAGGGA8950.017.34743519
Produced by FastQC (version 0.11.5)