FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n02_E2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n02_E2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27038120
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26850.012.9995342
GTAGAAC31650.011.22934211
GTACTCG61700.010.2527814
GCGTGAC41050.010.1490711
AATCGAA51300.09.5209115
TACTCGA64450.09.4641695
AGTACTC69250.09.3960313
GGAAAAT112150.09.3774611
TCAGGTA42900.09.228917
AAAATCC81450.08.7349893
GTGACTC43700.08.604023
GAATTGA67750.08.34036354
GAATCGA42500.08.2103814
CTCGAAT22600.08.0713151
AATTGAC45500.08.0632925
GGTAGAA47000.07.75338510
CGGCTAT33500.07.7307341
GAAAAAT132750.07.68477871
CTCTATA60500.07.658863545
CACTCGT58900.07.638515535
Produced by FastQC (version 0.11.5)