FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n02_E2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n02_E2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26738950
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC51900.011.91862111
TCAGGTA63000.010.3905847
CAGGTAG64800.09.7895068
TAGAACA65150.09.49479212
GGTAGAA67300.09.49222610
AATCGAA48850.08.9385285
AGGTAGA73850.08.9247929
TAATCGA31100.08.8311914
AATCGAG49950.08.6966235
CTAATCG24350.08.6914753
CTCGAAT25100.08.6131351
GGAAAAT136150.08.4686871
AGATCAG113150.08.0379754
GAATCGA42100.07.8071164
AAAATCC91600.07.7916313
GAATTGA74550.07.67018654
ATCAGGT109450.07.6252446
AACAGTG87900.07.422528715
GTACTCG34850.07.2995414
AGCATAA108750.07.1625344
Produced by FastQC (version 0.11.5)