FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n02_E2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n02_E2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28374358
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC39300.09.61578311
TCAGGTA49250.08.67849357
GTACTCG49100.08.6200614
CGTGACT25250.08.5615242
GGAAAAT116500.08.3895961
GAATCGA45150.08.2772154
TACTCGA53500.08.2038895
CTCGAAT23700.08.1719811
CACTCGT67700.07.742060735
GGTAGAA53300.07.59652110
CAGGTAG54000.07.58181338
AATCGAA53400.07.4605525
TAGAACA53600.07.42819512
AGAACGG170950.07.3887053
ACTCGTG72250.07.2546936
CCGTATG24500.07.161350345
GCGTGAC34300.07.1566471
GCCGTAT25850.07.13544844
GAATTGA72800.07.08170844
GGGAAAT64250.07.0803621
Produced by FastQC (version 0.11.5)