FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n02_E2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n02_E2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24105919
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC30400.010.65513211
CGTGACT19500.010.0466362
AATCGAA43350.08.9303765
TCAGGTA40750.08.6117477
GTACTCG38850.08.4022534
GGTAGAA40800.08.10460110
GCGTGAC28350.08.1024871
GAATTGA58700.08.0921344
GGAAAAT91650.08.0104391
CACTCGT60650.07.97453835
GTACGCA13000.07.95992048
TACTCGA41400.07.93748575
GCGGAAA71050.07.9240491
CTCGAAT20500.07.90951441
GAAAAAT119800.07.87641141
CAGGTAG46300.07.7737988
GAATCGA35650.07.7671974
TAGAACA43000.07.74227512
AGCCTAG20800.07.68126125
AGAACGG155950.07.5649363
Produced by FastQC (version 0.11.5)